Humoral responses to rFliC.

<p>ELISAs using HRP-conjugated rabbit anti-human IgM (A-C) and IgG (D-F) were conducted on prepared plates coated with 15 μg/ml of rFliC. Serum samples from 200 melioidosis patients were used at a dilution of 1:300. Box plots show OD at 450 nm for different groups of patients. All data in box plots are presented as 25^th and 75^th percentile boundaries in the box with the median line within the box; the whiskers indicate the 10^th and 90^th percentiles. Mann–Whitney test was used to assess for statistically significant difference of median OD values between different serum groups.