Distribution of Anti-Neu5Gc IgG titers in EPIC-Norfolk cohort using defined ELISA target antigens.

<p>Each dot represents a value for a single subject. (A) Levels of serum anti-Neu5Gc IgG quantified by ELISA using Neu5Gcα2-polyacrylamide (Neu5Gcα2-PAA) as the target antigen (N = 2716). The mean anti-Neu5Gc IgG titer was 8.6 μg/ml (SD±10.6). (B) Levels of serum anti-Neu5Gc IgG quantified by ELISA using Neu5Gcα2–6Lacβ-human serum albumin (HSA) as the target antigen (N = 2712). The mean anti-Neu5Gc IgG titer was 1.4 μg/ml (SD±1.2). (C) There was no correlation between anti-Neu5Gc IgG antibody levels directed against the two different chemically synthesized ELISA targets: Neu5Gcα2-PAA and Neu5Gcα2–6Lacβ-HSA.