Humoral immune responses following infection of rhesus monkeys with rRRV-SIVcmv-nfl.

<p>Sera obtained at the indicated time points following rRRV-SIVcmv-nfl inoculation were diluted 1:20 and tested for the presence of <b>A)</b> anti-RRV responses via an ELISA utilizing ELISA plates coated with purified wild type RRV lysate. Reactive antibodies were detected with a horseradish-peroxidase-conjugated anti-rhesus IgG antibody. <b>B)</b> Similarly, the sera were tested for the presence of anti-gp140 antibodies via an ELISA using recombinant SIVmac239 gp140 to coat the ELISA plates. Reactive antibodies were detected with a horseradish-peroxidase-conjugated anti-rhesus IgG antibody. <b>C)</b> The obtained sera were tested for the presence of anti-gp120 antibodies via an ELISA using recombinant SIVmac239 gp120 to coat the ELISA plates. Reactive antibodies were detected with a horseradish-peroxidase-conjugated anti-rhesus IgG antibody.