Additional file 2: of SNAI2 upregulation is associated with an aggressive phenotype in fulvestrant-resistant breast cancer cells and is an indicator of poor response to endocrine therapy in estrogen receptor-positive metastatic breast cancer

Figure S2. SOX2 knockdown reduces growth of fulvestrant-resistant breast cancer cells. (A) 182R-1 fulvestrant-resistant cells were transfected with siRNA against SOX2 leading to a reduction at the mRNA level, as evaluated by RT-qPCR. Gene expression was normalized using PUM1. (B) SOX2 knockdown resulted in decreased growth of fulvestrant-resistant cells as measured by crystal violet-based colorimetric assay. Cells were grown in medium containing fulvestrant. Experiment was performed in technical triplicates and results are shown with error bars representing mean ± standard deviation. (TIF 313 kb