Additional file 5: of Modelling invasive group A streptococcal disease using bioluminescence

Figure S5. Fitness burden conferred by bioluminescence in Galleria infection. Virulence of parent strains, M89 (blue line) and M75 (red line) was compared with bioluminescent derivatives M89::Lux (dotted blue line) and M75::Lux (dotted red line) in Galleria mellonella survival assays (n = 20 larvae per group). Larvae were inoculated with 1-2 × 10 6 cfu and monitored daily for survival over 5 days. Kaplan-Meier survival plots of infected larvae are shown compared with a PBS-inoculated group (controls – black line). Differences between strains were not significant. (PNG 38 kb