Nipah virus (NiV) replication and genome organization.

<p>(A) Nipah virus replication cycle. NiV enters a cell, where the viral genome is released, leading to the initiation of transcription and the accumulation of viral mRNA transcripts. In addition, the viral genome is transcribed into a full-length anti-genome, which is used to generate additional copies of the NiV genome. Viral mRNA transcripts are translated into viral proteins, leading to virion assembly, encapsidation, and virus release. (B) Schematic of NiV qRT-PCR assay. The NiV qRT-PCR assay was designed across the NiV F-G intergenic region. The forward primer binds to the untranslated region of the F gene. The reverse primer and hydrolysis probe bind to the 3’ region of the G gene, thereby preventing viral mRNA transcripts from providing false signals and artificially increasing viral RNA concentrations.