Immunostaining for pancreatic insulin-producing cells of rats after 4 months of treatment.

<p>(A) Insulin-producing cells index; (B) Total number of insulin-producing cells; (C) Detail of a pancreatic islet, typical of a non-diabetic animal, immunostained for insulin (200x magnification); (D) Detail of a pancreatic islet, typical of a diabetic animal, immunolabelled for insulin (200x magnification). Groups: control (CC), control with food restriction (CCR), diabetic+streptozotocin+cafeteria-style diet (DEC); diabetic+streptozotocin+cafeteria-style diet with food restriction (DER), diabetic+streptozotocin+nicotinamide (DNC) and diabetic+streptozotocin+nicotinamide with food restriction (DNR). Results expressed as mean±SE (n = 5/group). *a p<0.05 <i>vs</i> CC; b p<0.05 <i>vs</i> DEC; c p<0.05 <i>vs</i> DNC. One-way ANOVA and Tukey’s <i>post-hoc</i> test analysis.