Intestinal organoids from <i>G542X</i> mice are used to test PTC124 mediated readthrough.

<p>G524X organoids were treated with the indicated doses of PTC124 for 72 hours prior to the FIS assay. (A) Representative images at T = 0 and T = 300 minutes of organoid swelling, conditions as indicated. (Scale bar = 100 μm). (B) Normalized areas for PTC124 treated organoids are shown, compared to vehicle and 100 μM G418-treated organoids. (C) AUC measurements at 300 minutes. (*P<0.05 compared to untreated <i>G542X</i>.).