Additional file 1: of Systematic analyses reveal long non-coding RNA (PTAF)-mediated promotion of EMT and invasion-metastasis in serous ovarian cancer

Table S1. Statistics of datasets. Figure S1. LncRNA-EMT gene correlation network in mesenchymal ovarian cancer. Nodes marked by rounded rectangle and ellipse denote lncRNA and EMT gene, respectively. Nodes with blue or green color represent lncRNA or EMT genes are significantly up-regulated in mesenchymal OvCa compared with epithelial OvCa. The edges represent the significant correlation between lncRNAs and EMT genes. Figure S2. Overexpression of miR-25 in OvCa cells promotes epithelial phenotype. (A) The mRNA levels of EMT-related markers in SKOV3 cells transfected with miR-25 or control miRNA (miR-Ctrl). (B) Quantification of E-cadherin and Vimentin in SKOV3 cells transfected with miR-25 or control miRNA (miR-Ctrl). Wound healing assay (C) and migration assay (D) determined the effect of miR-25 on cell migration in OvCa cells. n = 5 independent experiments. *p < 0.05, **p < 0.01 vs. miR-Ctrl. Figure S3. PTAF promoted migration in SKOV3 cells. (A) Overexpression of PTAF induced EMT by regulating miR-25 in SKOV3 cells, as measured by qRT-PCR. n = 6 independent experiments. *P < 0.05. Wound-healing (B) and migration assays (C) showed that PTAF promoted SKOV3 cell migration, which was inhibited by forced expression of miR-25. n = 5 independent experiments. *P < 0.05, **P < 0.01. (D) A Transwell invasion assay showed that PTAF promoted SKOV3 cell invasion by egulating miR-25. n = 5 independent experiments. **P < 0.01. Figure S4. Silencing of PTAF inhibited TGF-driven migration in SKOV3 cells. (A) Knockdown of PTAF attenuated TGF-β1-induced EMT in SKOV3 cells, which could be alleviated by miR-25 inhibition. n = 6 independent experiments. *P < 0.05. Wound-healing (B) and migration assays (C) showed that silencing of PTAF inhibited TGF-β1-driven migration in SKOV3 cells, which was abated by miR-25 knockdown. n = 5 independent experiments. *P < 0.05, **P < 0.01. (D) A Transwell invasion assay showed that knockdown of PTAF inhibited TGF-β1-induced SKOV3 cell invasion. n = 5 independent experiments. **P < 0.01. (DOC 13561 kb