A2AR agonist is more inhibitory in the absence of γδ T cells.

<p>A) A2AR agonist inhibited TCR-δ^-/- responder T cells more effectively than it inhibited the B6 responder T cells. The αβ responder T cells (1 x 10⁶/well) were isolated from IRBP_1-20-immunized B6 (upper panels) or TCR-δ^-/- mice (lower panels). The CFSE-labeled αβ T cells were stimulated for 5 d with immunizing peptide and APCs in the absence or presence of A2AR agonist. Cell proliferation was assessed by FACS analysis. B) In vitro treatment of A2AR agonist enhanced the Th17 responses when added to B6 responder T cells but inhibited the Th17 responses when added to TCR-δ^-/- responder T cells. Comparison of IL-17 and IFN-γ production by immunized αβ T cells isolated from immunized B6 or TCR-δ^-/- mice after in vitro stimulation, in the absence or presence of A2AR agonist, showing that A2AR agonist inhibited the Th1 response but enhanced the Th17 response of B6 cells, but not of TCR-δ^-/- T cells.