Cell surface phenotype of naïve and T_SCM populations.

<p>(A) Gating strategy used to sort CD4⁺ and CD8⁺ naïve and T_SCM cells for isotope label and telomere length analysis: the top panels show the consecutive gating to detect CD8⁺ or CD4⁺ T cells; the bottom panels show the further gating to detect naïve or T_SCM cells within CD8⁺ or CD4⁺ populations. (B) Expression of CD45RO, CD28, CD127, and CD45RA on CD8⁺ CCR7⁺ CD95⁻ naïve cells (blue cloud) and CD8⁺ CCR7⁺ CD95⁺ T_SCM cells (red cloud) compared with bulk CD8⁺ T cells (black cloud). (C) as for B but depicting CD4⁺ naïve and CD4⁺ T_SCM compared to bulk CD4⁺ T cells. FSC-A, forward scatter area; FSC-H, forward scatter height; SSC-A, side scatter area; T_SCM, stem cell–like memory T.