Binding and neutralizing potentials of MAbs isolated from the NLGS-3 Core immunized animals.

<p>(A) Binding (<i>top</i>) and neutralizing (<i>bottom</i>) titers of the indicated macaque MAbs against the indicated Envs and viruses. (B) Antibody-binding competition. Top panels: The binding of the indicated macaque MAbs to the 426c NLGS-3 Core gp140 protein was determined in the absence of competing antibody <i>(left)</i>, or following the pre-incubation of 426c NLGS-3 Core gp140 with saturating concentrations of VRC01 <i>(middle left)</i>, CD4-IgG <i>(middle right)</i>, or b12 <i>(right)</i>. Bottom panels: Control experiments were performed with the indicated human MAbs. Direct binding of the indicated human MAbs to the 426c NLGS-3 Core gp140 protein (<i>left panel</i>), following the pre-incubation of Env with VRC01 (<i>second from left panel</i>), CD4-IgG (<i>second from right panel</i>), or b12 (<i>right panel</i>).