MOESM9 of Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids

Additional file 9: Figure S9. Overexpression of an LD-associated protein-encoding gene TadA decreased the TAG titer in strain 2119. Cells were cultured in ZYP-5052 auto-induction medium at 37°C with shaking at 200 rpm for 48 hr. Lipids extracted from 5 mg dried cells were loaded on TLC plate. Strain 2119, E.coli BL21(DE3) harboring pCDFDuet::tDGAT/RoPAP::RoFadD1/RoTetR2