Deletion of the <i>SIW14</i> phosphatase gene results in exaggerated pseudohyphal growth.

<p><b>(A)</b> Surface pseudohyphal filamentation phenotypes of homozygous diploid strains with the indicated InsP phosphatase mutations. Strains were grown on low-nitrogen SLAD medium. Exaggerated pseudohyphal growth in the <i>siw14</i>Δ/Δ mutant is indicated with a “++”; wild-type pseudohypha growth levels are indicated with a “+”. Pseudohyphal growth assays were performed and quantified as described; mean values with standard deviation for three replicates are indicated. Scale bar, 500 μm. <b>(B)</b> Representative InsP profiles of mutated InsP phosphatase strains grown in low-nitrogen minimal media for eight hours. The proportional CPM counts are shown to 1% in order to indicate the changes in IP₆ levels. The InsP trace for a wild-type control strain is shown below the plot. Ratios of 5PP-InsP₅:1PP-InsP₅ are indicated as mean with standard deviation. Ratios of InsP₇ in individual replicates are listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007493#pgen.1007493.s011" target="_blank">S7 Table</a>.