DNA replication parameters in RecQ helicase mutants.

<p>(A) Representative DNA fibres from synchronous blood-stage parasites. Upper panel: DNA fibres are in blue, IdU in red and CldU in green; lower panel: the IdU and CldU tracks extracted from the upper panel. 50 kb scale bars are indicated. (B, C and D) Comparative analysis of replication fork speed (B) inter-origin distances (C) and asymmetric forks (long fork to short fork ratios) (D) from synchronous blood-stage parasites. Black bars on dot plots indicate median values. The two-tailed Mann-Whitney test was used to calculate the corresponding P values (ns, not statistically significant P value; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001). (E) Percentage of symmetric, asymmetric and unidirectional replication forks counted in the wildtype and RecQ helicase mutants. The groups were significantly different by Chi-square test (P < 0.00001).