Development and architecture of colonies with altered levels of Cyc8p and Tup1p.

<p>A, Development of colonies grown on GMA at a density of ~10³ colonies per plate (left panel). Two photon excitation confocal microscopy (2PE-CM) of colony cross-sections stained with Calcofluor white (false green color); colonies were grown for 5 days on GMA at a density of ~3 x 10³ colonies per plate (right panel). B, 2PE-CM of colony cross-sections stained with Calcofluor white (false green color). Colonies were grown for 3 days on GMA at a density of ~3 x 10³ colonies per plate. Upper part, whole colonies (20x objective); lower part, central colony parts shown at a higher magnification (63x objective), insets: details of aerial and subsurface cells (strains BR-F and p_GAL-<i>CYC8</i>); detail of central part (p_TEF-<i>CYC8</i>); detail of the colony bottom (<i>tup1</i>). White bar, 100 μm; yellow bar, 20 μm. Arrow indicates chains of rounded cells invading the agar.