Functional mode analysis.

<p>(A) Amino acids contributing to the motions correlated with occupancy variations of H-bonds spanning residues G33-V42. Red stars indicate hinge residues. The percentage of helix bending is shown with red bars in the right part (%-twisting amounts to 100 - %-bending). Black bars quantify similarities between mutants and WT. (B) Backbone representation of structures interpolating from low to high occupancies along the motions from (A). (C) The same as for (A) for H-bonds spanning residues V40-I47. Hinge residues are highlighted by red and blue stars; percentages of helix bending are shown with accordingly coloured bars. (D) Backbone representation of structures interpolating from low to high occupancies along the motions from (C). For the graphical representations, structures have been overlaid onto the A30-L34 segment. The part of the backbone spanned by the corresponding H-bonds is coloured in yellow. Functional modes were clustered, the depicted cluster representative is indicated with bold letters. ε-cleavage sites (T48, L49) are shown as red and blue spheres, respectively.