Figure S1. Antibody profiling of B. microti proteins by protein arrays. Crude B. microti proteins (2D3-2D54, 2D55-2D128) react with plasma samples across ten different time points (a-j: 0, 3, 7, 14, 21, 30, 60, 120, 150 and 270 dpi). The reactions were detected with anti-mouse IgG (A) and anti-mouse IgM (B). Control reactions of wheat germ lysate that lacked vector templates (white box) and reactions of purified recombinant proteins (red box) served as negative and positive controls. Well characterized reactions with target proteins are marked with orange boxes. (TIF 3404 kb
Cell-mediated immunity of mice immunized with recombinant MSAs. C57BL/6 mice were immunized as descr...
Figure S1. Intraerythrocytic Babesia bovis merozoites express RON2. Merozoites were incubated with b...
Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (...
Table S6. Babesia microti proteins were grouped by immunoreactivity patterns. (DOCX 17 kb
Figure S3. Two hundred febrile cases evaluated by crude B.microti proteins and recombinant antigens....
Figure S2. Soluble expression analysis of rBm2D97, rBm2D33, rBm2D41 and rBm7. M: MW markers; Lane 1:...
Figure S4. Evaluating febrile cases using nested-PCR and recombinant proteins by ELISA. (TIF 6386 kb
Table S4. The primer sequences of B. microti genes in prokaryotic cloning and expression. (DOCX 14 k...
Abstract Background Babesiosis is caused by the invasion of erythrocytes by parasites of the Babesia...
SDS-PAGE analysis of purified recombinant proteins. Proteins were expressed in E. coli and purified ...
Excellent analytical specificity of the SBP4 MI-ELISA was demonstrated by no cross-reactivity agains...
Abstract Babesia microti is an intraerythrocytic parasite and the primary causative agent of human b...
Additional file 1: Figure S1. Localization of BoTRAP2 on B. orientalis by indirect immunofluorescenc...
Table S1. Primers used for cloning. Underlining indicates the recognition sites of restriction enzym...
A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of ...
Cell-mediated immunity of mice immunized with recombinant MSAs. C57BL/6 mice were immunized as descr...
Figure S1. Intraerythrocytic Babesia bovis merozoites express RON2. Merozoites were incubated with b...
Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (...
Table S6. Babesia microti proteins were grouped by immunoreactivity patterns. (DOCX 17 kb
Figure S3. Two hundred febrile cases evaluated by crude B.microti proteins and recombinant antigens....
Figure S2. Soluble expression analysis of rBm2D97, rBm2D33, rBm2D41 and rBm7. M: MW markers; Lane 1:...
Figure S4. Evaluating febrile cases using nested-PCR and recombinant proteins by ELISA. (TIF 6386 kb
Table S4. The primer sequences of B. microti genes in prokaryotic cloning and expression. (DOCX 14 k...
Abstract Background Babesiosis is caused by the invasion of erythrocytes by parasites of the Babesia...
SDS-PAGE analysis of purified recombinant proteins. Proteins were expressed in E. coli and purified ...
Excellent analytical specificity of the SBP4 MI-ELISA was demonstrated by no cross-reactivity agains...
Abstract Babesia microti is an intraerythrocytic parasite and the primary causative agent of human b...
Additional file 1: Figure S1. Localization of BoTRAP2 on B. orientalis by indirect immunofluorescenc...
Table S1. Primers used for cloning. Underlining indicates the recognition sites of restriction enzym...
A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of ...
Cell-mediated immunity of mice immunized with recombinant MSAs. C57BL/6 mice were immunized as descr...
Figure S1. Intraerythrocytic Babesia bovis merozoites express RON2. Merozoites were incubated with b...
Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (...