Additional file 2: of Administration of cytokine-induced myeloid-derived suppressor cells ameliorates renal fibrosis in diabetic mice

Figure S4. Glucose, not d-mannitol, reduced MDSC production. BM cells from femur and tibia of B6 mice were cultured in presence of mouse recombinant GM-CSF (10Â ng/ml) under normal or high glucose conditions with or without 20Â mM of d-mannitol. Cells isolated for examination 7Â days later. Isolated cells two-color stained with specific mAbs against CD11b and Gr-1 for flow analyses (PDF 111 kb