Additional file 1: of Ablation of tau causes an olfactory deficit in a murine model of Parkinson’s disease

Supplementary data Table S1. Animal numbers (genotype and sex). Table S2. ODT ANOVA factors. Normality test: Shapiro-Wilk. Table S3. ODT one sample t test, hypothetical mean = 50% (chance). Figure S1. ODT (A) and motor evaluation (B) of 12-month-old tau−/− (n = 12) and WT (n = 12). ODT analysed by two-way repeated measures ANOVA (one factor repetition) with Fisher LSD post-hoc comparisons. # represents significant main effect of genotype, ## p < 0.01. Motor tests analysed by unpaired two-sided t test. Ns = not significant. Figure S2. Western blot confirmation of tau ablation in 7- and 15-month old tissue. A) Tau antibody (Dako, catalogue number: A0024, dilution 1:10,000) confirmed tau ablation B) Protein loading in all wells confirmed by GAPDH (Cell Signaling Technology, catalogue number 2118, dilution 1:10,000). Figure S3. Full Western Blots of 7 mo WT (+) and Tau−/− (−) cell lysate. A, B, C: immunoblot of p62 from OB, CPU and SN respectively. D, E, F: immunoblot of LCB3 from OB, CPU and SN respectively. G, H, I: immunoblot of α-synuclein from OB, CPU and SN respectively. Red box indicates the section represented in Figures. Figure S4. Full Western Blots of 15 mo WT (+) and Tau−/− (−) cell lysate. A, B, C: immunoblot of p62 from OB, CPU and SN respectively. D, E, F: immunoblot of LCB3 from OB, CPU and SN respectively. G, H, I: immunoblot of α-synuclein from OB, CPU and SN respectively. Red box indicates the section represented in Figures. Figure S5. A) Full Western Blots of PCN for LC3B from WT (+) and Tau−/− (−) cell lysate. B) Full Western Blot of PCN-derived exosomes for α-synuclein from WT (+) and Tau−/− (−) cells. C) Exosome verification using exosome negative markers (GM130 & bcl-2), exosome positive marker (Tsg101) and microvesicle marker (Flotillin) in primary cortical neuron cell lysate and exosomes. (PDF 1104 kb