Bat fly-associated Laboulbeniales log and trees files LSU

Output log and .trees files of the BEAST analysis of the LSU dataset, under a TPM2uf+G model of nucleotide substitution. Two independent runs were performed for 40 million generations.<div><br></div><div><br></div><div><div>--</div><div>Haelewaters et al. 2018. Laboulbeniales hyperparasites (Fungi, Ascomycota) of bat flies: Independent origins and host associations. DOI:10.1002/ece3.4359</div><div><br></div><div>ABSTRACT</div><div>The aim of this study was to explore the diversity of ectoparasitic fungi (Ascomycota, Laboulbeniales) that use bat flies (Diptera, Hippoboscoidea) as hosts. Bat flies themselves live as ectoparasites on the fur and wing membranes of bats (Mammalia, Chiroptera); hence this is a tripartite parasite system. Here, we collected bats, bat flies, and Laboulbeniales, and conducted phylogenetic analyses of Laboulbeniales to contrast morphology with ribosomal sequence data. Parasitism of bat flies by Laboulbeniales arose three times independently, once in the Eastern Hemisphere (Arthrorhynchus) and twice in the Western Hemisphere (Gloeandromyces, Nycteromyces). We hypothesize that the genera Arthrorhynchus and Nycteromyces evolved independently from lineages of ectoparasites of true buge (Hemiptera). We assessed phylogenetic diversity of the genus Gloeandromyces by considering the LSU rDNA region. Phenotypic plasticity and position-induced morphological adaptations go hand in hand. Different morphotypes belong to the same phylogenetic species. Two species, G. pageanus and G. streblae, show divergence by host utilization. In our assessement of coevolution, we only observe congruence between the Old World clades of bat flies and Laboulbeniales. The other associations are the result of the roosting ecology of the bat hosts. This study has considerably increased our knowledge about bats and their associated ectoparasites and shown the necessity of including molecular data in Laboulbeniales taxonomy. </div><div><br></div><div>KEYWORDS</div><div>Ascomycota, ectoparasites, host specialization, phenotypic plasticity, ribosomal DNA, taxonomy</div>