Tumor growth kinetics in mouse chimeras.

<p>Lymphocytes isolated from C57Black/6 mice were transplanted into RAG1^-/- mice previously injected with 5x10⁴ TC-1 cells. One to 3 million lymphocytes were transplanted per mouse. When mixed, B or DC and T lymphocytes were transplanted in a 1:1 ratio. (A) T lymphocytes from tumor bearing donors, Tumor T, were injected alone or with B lymphocytes from tumor bearing donors activated with anti-CD40 or control: /CD40 tumor B or /control tumor B. (B) T cells from tumor bearing donors, Tumor T, were injected alone or with B cells from naïve donors, activated with anti-CD40 or control: /CD40 naive B or /control naive B. (C) T lymphocytes from naïve donors, Naïve T, were injected into the recipients, alone or with B lymphocytes from tumor bearing donors activated with anti-CD40 or control: /CD40 tumor B and /control tumor B. Experimental groups of 4 to 5 mice (the experiments were repeated 2 times, so a total of at least 8 to 10 mice were tested). Differences between groups was tested by Mann-Whitney U test, the tumor growth kinetics had experimental groups of at least 6 mice; * indicates p<0.05. Significant differences between groups had the same pattern when tested by ANOVA using as data the areas under the curves.