Correlation between EphA2 expression level and infection rates.

<p><b>A.</b> Hepa 1–6 cells infected with PbGFP sporozoites were stained 24 hours post-infection with an anti-EphA2 primary antibody (D4A2) followed by Alexa Fluor 594 secondary antibodies (blue histogram), and analyzed by FACS. The grey histogram corresponds to control cells labeled with secondary antibodies only. The vertical line indicates the threshold used to distinguish EphA2^low and EphA2^high cells. <b>B.</b> The percentage of EphA2^low and EphA2^high cells, was determined in non-infected (GFP-negative) and infected (GFP-positive) cells. <b>C.</b> Hepa 1–6 cells infected with PbGFP sporozoites were stained 24 hours post-infection with an anti-CD81 primary antibody (MT81) followed by Alexa Fluor 594 secondary antibodies (blue histogram), and analyzed by FACS. The grey histogram corresponds to control cells labeled with secondary antibodies only. The vertical line indicates the threshold used to distinguish CD81^low and CD81^high cells. This threshold was arbitrarily defined to obtain a majority of CD81^low population after siRNA silencing of CD81. <b>D.</b> The percentage of CD81^low and CD81^high cells, was determined in non-infected (GFP-negative) and infected (GFP-positive) cells. <b>E.</b> Hepa1-6 cells were transfected with siRNA targeting CD81 and infected with PbGFP sporozoites. Cells were stained 24 hours post-infection with an anti-CD81 primary antibody (MT81) followed by Alexa Fluor 594 secondary antibodies (orange histogram), and analyzed by FACS. The grey histogram corresponds to control cells labeled with secondary antibodies only, and the blue histogram corresponds to the control cells labeled with CD81 antibodies, as in C. The vertical line indicates the same threshold as in C, used to distinguish CD81^low and CD81^high cells. <b>F.</b> The percentage of CD81^low and CD81^high cells was determined in non-infected (GFP-negative) and infected (GFP-positive) CD81 siRNA-treated Hepa1-6 cells.