p38α in human visceral fat inversely correlated with BMI and directly correlated with UCP1 in human visceral fat and sWAT.

<p>(A) mRNA levels of <i>Mapk14</i> (p38α) in visceral fat from lean individuals and individuals with obesity—mRNA expression was normalised to the amount of <i>Gapdh</i> mRNA. (B) Correlation between mRNA levels of <i>Mapk14</i> (p38α) and BMI (r² = −0,365; <i>p</i> = 0.001) or (C) <i>Ucp1</i> in visceral fat (r² = 0.316; <i>p</i> = 0.007). The mRNA levels of <i>Mapk14</i> (p38α) and <i>Ucp1</i> were determined by qRT-PCR (<i>n</i> = 71). (D) mRNA levels of <i>Mapk14</i> (p38α) in sWAT from lean individuals and individuals with obesity. mRNA expression was normalised to the amount of <i>Gapdh</i> mRNA. (E) Correlation between mRNA levels of <i>Mapk14</i> (p38α) and <i>Ucp1</i> in sWAT (r² = 0.320; <i>p</i> < 0.0001). Graph correlating mRNA <i>Mapk14</i> and log mRNA <i>Ucp1</i> is also shown. The mRNA levels of <i>Mapk14</i> (p38α) and <i>Ucp1</i> were determined by qRT-PCR (<i>n</i> = 168). See also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004455#pbio.2004455.s015" target="_blank">S1 Data</a>. Linear relationships between variables were tested using Pearson’s correlation coefficient. BMI, body mass index; qRT-PCR, quantitative real-time polymerase chain reaction; UCP1, uncoupling protein 1.