Comparative growth curve analyses of three AF and three AF ZIKV strains in ESCd, JAr, and Vero cells.

<p>Cells were infected with the six ZIKV strains at a MOI of 0.1. Cell supernatants were harvested at the indicated time points for titration by plaque assay in Vero cells. Growth curve analyses were performed in triplicate and plotted as SEM. Growth curves representing the AF strains are highlighted in shades of blue and those representing the AS strains are shown in shades of red. (A) In ESCd, significantly higher virus concentrations were observed by 48 h PI for the AF Uganda and the AF Senegal strains when compared to all other strains (<i>p</i> < 0.05). No significant differences were observed between the Nigeria, Cambodia, Mexico, and Panama strains. (B) In JAr cells, significantly higher concentrations were observed by 48 h PI for the AF Uganda strain when compared to all other strains (<i>p</i> < 0.001). No significant differences were observed between the Senegal, Nigeria, Cambodia, Mexico, and Panama strains. (C) In Vero cells, significantly higher virus concentrations became obvious by 48 h PI for the AF Uganda, the AF Senegal, and the AS Mexico strains when compared to the other three viruses (<i>p</i> < 0.01). No significant differences were observed between the Nigeria, Cambodia and Panama strains.