Cytopathic effects (CPE) caused by three AF and three AS ZIKV strains in JAr cells.

<p>(A) Two separately cultured groups of JAr cells were exposed continuously to either enzymatic dispersal (>10 passages, trypsin) or non-enzymatic dispersal (gentle disassociation reagent, STEMCELL Technologies). Prior to infection, each group was dispersed by a non-enzymatic agent, to allow uniform cell dispersal. Two days after plating, the groups were infected with the AF Uganda strain of ZIKV at an MOI of 0.27. At 48 hours PI, live cell images were captured to demonstrate CPE (bottom panel). Cells were fixed and stained with crystal violet (top panel). Areas showing clear CPE are highlighted by red rectangles. (B) JAr cells were infected with each ZIKV strain at 1 MOI and fixed either at 48 h or 60 h PI. Respective mock infected controls are shown below. Lysis is only evident after infection with the three AF strains of ZIKV. Scale bars are 100 μm for live cell images in (A) and 1 mm in (A) and (B) for crystal violet stained images.