Therapeutic NSCs delivered within the resection cavity regress and delay re-growth of post-surgical MB.

<p>White light and fluorescent images of NSC expressing TK (NSC^tk) in culture (A, B) or mixed with fibrin matrix (NSC^tk/fibrin) (C, D), illustrate high transduction efficiency and efficient expression of the construct. Depiction of the <i>in vivo</i> therapy schema, in which tumors were grafted within the cerebellum, resected and treated with therapeutic NSC^tk/fibrin (E). Representative white light (F) and fluorescent (G) images confirm NSC^tk/fibrin implanted within the resection cavity. NSC^tk/fibrin persist longer in the brain without systemic administration of GCV (NSC^tk) compared to with systemic administration of GCV (NSC^tk/GCV) (H). Representative BLI images of NSC^tk and NSC^tk/GCVmice at days 3 and 7 post-surgery are shown above. Daoy-GFPFL cells were seeded with varying concentrations of NSC^tk (I). Tumor cell viability was measured by luminescence 24h after GCV administration. Summary graph and representative BLI show a significant reduction of tumor burden in NSC^tk/fibrin+GCV-treated mice compared to control-treated (J; no NSC and NSC^tk). NSC^tk/fibrin with GCV treatment extended median survival two-fold compared to controls (9 vs. 21 days, log rank *P<0.0001, K). Original magnification: 40X (C, D), 100X (A, B). Scale bars = 100 μm. n = 4 per group in the panel H, n = 4 in no NSC and NSC^TK, n = 7 in the NSC^TK/GCV group in J-K.