Non-lethal effects (mean±S.E.) on <i>Ostrinia nubilalis</i> larvae fed from second instar to pupation on a diet made with kernels of different maize lines.

<p>4Bt contained the Cry1Ac toxin, HC was the transformed line with high carotenoid content, M37W was the near isogenic line, and 4BtxHC was the line with the two traits. <b>a)</b> Percentage of pupation [F = 541.27; P<0.001; df = 3,156]. <b>b)</b> pupal weight [F = 34.04; P<0.001; df = 2,56]. (g) <b>c)</b> Larval development from bioassay initiation to pupation [F = 98.16; P<0.001; df = 2,56]. and <b>d)</b> Pupal size of <i>O</i>. <i>nubilalis</i> larvae fed for four weeks on a diet made with grains of M37W (control, left figure), HC (middle), and 4BtxHC (right figure) plants. Different letters indicate statistically significant differences between the lines (P< 0.001) when data were analysed by one-way (plant line) ANOVA.