Figure S4. Overlaid 1H-15N HSQC spectra of TPR-CT∆Q SGTA at a range of temperatures from 5 °C (gray-blue) to 40 °C (maroon). (PDF 3684 kb
Figure S1. Sequence alignment of human SGTA (Homo sapiens) and several homolog proteins: Sumatran or...
Figure S14. DEER measurements and distances determined for FL and NT-TPR SGTA constructs spin-labele...
RLI was supported by MRC New Investigator Research Grant: G0900936. RLI and SH are funded by BBSRC g...
Figure S9. Overlaid 1H-15N HSQC spectra of different SGTA constructs under the same conditions. (A) ...
Figure S5. Complete analysis of the chemical shift difference between SGTA constructs for the same b...
Figure S10. 15NÂ - {1H} heteronuclear NOE measurements of different SGTA constructs; in all cases, t...
Figure S8. Mass spectrometry analysis of SGTA CT construct, showing that the protein is not covalent...
Table S1. Detailed SAXS data collection and derived parameters for FL and NT-TPR constructs of SGTA....
Figure S6. Chemical shift index analysis of full-length SGTA, showing the alpha carbon and carbonyl ...
Figure S7. (A) Size-exclusion chromatography of some different variants of SGTA. Note the unexpected...
Figure S13. CW-EPR spectra of the FL and C-terminal deleted (NT-TPR) SGTA proteins. Room Temperature...
Figure S11. 15N NMR relaxation analysis of N-terminal (A) and TPR (B) domains in different SGTA cons...
Figure S12. Selected mutations in the TPR domain for MTSL labelling in the EPR experiments. (A) Cart...
Figure S3. SGTA-3xNNP/AAA-V5-expressing cells form cytosolic inclusions in the absence of an MLP sub...
Figure S2. The SGTA-3xNNP/AAA-V5 mutant stimulates the accumulation of OP91 in discrete cytosolic in...
Figure S1. Sequence alignment of human SGTA (Homo sapiens) and several homolog proteins: Sumatran or...
Figure S14. DEER measurements and distances determined for FL and NT-TPR SGTA constructs spin-labele...
RLI was supported by MRC New Investigator Research Grant: G0900936. RLI and SH are funded by BBSRC g...
Figure S9. Overlaid 1H-15N HSQC spectra of different SGTA constructs under the same conditions. (A) ...
Figure S5. Complete analysis of the chemical shift difference between SGTA constructs for the same b...
Figure S10. 15NÂ - {1H} heteronuclear NOE measurements of different SGTA constructs; in all cases, t...
Figure S8. Mass spectrometry analysis of SGTA CT construct, showing that the protein is not covalent...
Table S1. Detailed SAXS data collection and derived parameters for FL and NT-TPR constructs of SGTA....
Figure S6. Chemical shift index analysis of full-length SGTA, showing the alpha carbon and carbonyl ...
Figure S7. (A) Size-exclusion chromatography of some different variants of SGTA. Note the unexpected...
Figure S13. CW-EPR spectra of the FL and C-terminal deleted (NT-TPR) SGTA proteins. Room Temperature...
Figure S11. 15N NMR relaxation analysis of N-terminal (A) and TPR (B) domains in different SGTA cons...
Figure S12. Selected mutations in the TPR domain for MTSL labelling in the EPR experiments. (A) Cart...
Figure S3. SGTA-3xNNP/AAA-V5-expressing cells form cytosolic inclusions in the absence of an MLP sub...
Figure S2. The SGTA-3xNNP/AAA-V5 mutant stimulates the accumulation of OP91 in discrete cytosolic in...
Figure S1. Sequence alignment of human SGTA (Homo sapiens) and several homolog proteins: Sumatran or...
Figure S14. DEER measurements and distances determined for FL and NT-TPR SGTA constructs spin-labele...
RLI was supported by MRC New Investigator Research Grant: G0900936. RLI and SH are funded by BBSRC g...