Notch pathway activation in Ik^L/L pDCs.

<p><b>(A)</b><i>Hes1</i> and <i>Ptcra</i> mRNA expression in BM pDCs from 4 WT and 5 (Ik^L/L) mice, as analyzed by RT-qPCR and normalized to <i>Hprt</i> mRNA levels (mean±SD of triplicate data). (<b>B)</b> GFP reporter expression (black line) in total BM cells and BM pDCs (CD11c⁺CD317⁺) from Hes1-GFP⁺ WT and Ik^L/L mice, by flow cytometry. Grey histograms correspond to control cells from mice lacking the Hes1-GFP reporter. <b>(C)</b> Percentage of GFP⁺ BM pDCs (CD11c⁺CD317⁺) from Hes1-GFP⁺ WT and Ik^L/L mice, as analyzed in (B). *p≤0.05 (Student’s t-test). <b>(D)</b> CCR9 and SiglecH vs. GFP expression in BM pDCs from Hes1-GFP⁺ WT and Ik^L/L mice. Representative of 3 independent experiments.