Additional file 1: of BMP4 promotes hepatocellular carcinoma proliferation by autophagy activation through JNK1-mediated Bcl-2 phosphorylation

Figure S1. Original blots of Western blot. Original blots of Fig. 1. (2) Original blots of Fig. 2. (3) Orinigal blots of Fig. 3. (4) Orinigal blots of Fig. 4. (5) Orinigal blots of Fig. 5. (6) Orinigal blots of Fig. 6. Figure S2. The effeciency of BMP4 expression manipulated by lentivirus. The effeciency of BMP4 overexpression in HepG2 cells was confirmed by qRT-PCR and Western blot. (b)The effeciency of BMP4 knockdown in HCCLM3 cells was confirmed by qRT-PCR and Western blot. Figure S3. BMP4 promoted HepG2 cells growth. (a) HepG2 cells were treated with various concentrations of human recombinant BMP4 (0, 25, 50, 100 and 150 ng/mL) for different lengths of time to assay the effects on HCC cells proliferation. Cell viability was determined by CCK-8 assays. (b) & (c) Effects of BMP4 or Noggin on long term colony formation in HepG2 cells. The numbers of colonies in the BMP4-treated (100 ng/mL) groups were significantly more than that in the blank groups in HepG2 cells (p < 0.001), while Noggin-treated (200 ng/mL) groups displayed significantly less colony numbers than the blank control groups (p < 0.05). n = 3, one-way ANOVA with post-hoc Tukey’s test. Table S1. Information of antibodies for western blot. Table S2. The sequences of siRNA targeting BMP4. (PDF 783 kb