Detection of miR iab-8 using a GFP sensor.

<p>Dissected accessory glands from <b><i>A</i>.</b> a fly carrying an mCherry reporter in the <i>msa</i> second exon (<i>exon2-mCherry</i>), <b><i>B</i>.</b> a wild type fly carrying a <i>mir iab-8</i> GFP sensor and <b><i>C</i>.</b> an <i>iab-6</i>^{<i>cocuD1</i>} homozygous fly in the presence of a <i>mir iab-8</i> GFP sensor (22)<b>. <i>C’</i>.</b> is an enlargement of the box shown in <b><i>C</i>.</b> In <b><i>A</i>.</b>, mCherry is visualized directly, while in B., C. and C’. GFP is visualized. <b><i>B</i>.</b> In wild-type AGs, secondary cells appear as non-GFP expressing holes in the plane of GFP-expressing main cells (examples shown with white arrows). <b><i>C</i>.</b> In the <i>iab-6</i>^{<i>cocuD1</i>} homozygous mutants, the secondary cells express GFP (examples shown with orange arrows). B’ is an enlargement of the orange box in B. The enlargement of the secondary cells allows for the visualization of the abnormal morphological phenotype (lack of large vacuoles) of <i>iab-6</i>^{<i>cocuD1</i>} secondary cells in the sensor line. Scale bar equals 50μm.