Association between CD161⁺ T cells and Th17 cells.

<p>PBMCs were stained with anti-CD4 PE-cy7, anti-CD161 APC, and anti-IL-17 PE antibodies. Lymphocytes were gated for further analysis. (A) Proportion (%) of CD161⁺/CD4⁺ IL-17⁺ T cells. (B) Proportion (%) of IL-17⁺/CD4⁺ CD161⁺ T cells in PBMCs. (C) PBMCs were cultured for 48 hours under Th0 differentiation conditions and the proportion (%) of CD161⁺/CD4⁺ IL-17⁺ T cells in PBMCs was analyzed. (D) PBMCs were cultured for 48 hours under Th17 differentiation conditions and the proportion (%) of CD161⁺/CD4⁺ IL-17⁺ T cells in PBMCs was analyzed. (E) The proportion (%) of CD161⁺/CD4⁺ T cells showed a significant positive correlation with the proportion (%) of IL-17⁺/CD4⁺ T cells (P = 0.02, r2 = 0.16). *<i>p</i><0.05 for each comparison.