MOESM1 of Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Additional file 1: Table S1 and S2. Ratings from one-way ANOVA analyses of initial reaction rates by endomannanases on pure mannans (Fig. 2). A one-way ANOVA analysis was made for all endomannanases on each substrate (top, Table S1) and for each enzyme across all substrates (bottom, Table S2). Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Table S3. Ratings from one-way ANOVA analyses of sugar release during softwood saccharification at 30 °C (Fig. 3 and Additional file 1: Figure S2). A one-way ANOVA analysis was made for each sugar at each time point. Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Table S4. Ratings from one-way ANOVA analyses of glucose release during softwood saccharification at 30 and 50 °C (Fig. 4). A one-way ANOVA analysis was made for each temperature at each time point. Ratings are assigned with a 95 % confidence interval with the Tukey–Kramer method in SASjmp. Please consult the manuscript for enzyme abbreviations. Figure S1. Stability of endomannanases at 30 °C. The relative activity (%) was determined on locust bean gum at 30 °C, pH 5 and are given as mean values ± SD (n=3). Please consult the manuscript for enzyme abbreviations. Figure S2. Softwood saccharification. Endomannanases or BSA were added in equal molar amounts on top of Cellic ® CTec3 plus an A. niger GH2 β-mannosidase (BM2). Samples were taken after 24, 48 and 144 h saccharification at 30 °C. Glucose (g/l, light grey), mannose (dark grey) and xylose (black) yields are given as mean values ± SD (n=3). One-way ANOVA analyses can be seen in Additional file 1: Table S3. Figure S3. SDS-PAGE gels of the purified enzymes. The protein concentration in the samples was 0.5 mg/ml. Prior to gel loading, samples were diluted 1:1 with loading mix. Loading mix was prepared as a 9:1 mix of Novex ® Tris-Glycine SDS Sample Buffer (2X) (Life Technologies) and Nupage ® Sample Reducing Agent (10X) (Life Technologies). Please consult the manuscript for enzyme abbreviations. Samples with NdesMan26A and MtheMan26A both contain molecules with and without the CBM35