<i>gcsfa</i> overexpression does not improve the survival of NOX incompetent larvae during notochord infection.

<p>(A) Diagram shows the experimental strategy to induce high neutrophil number during NOX inhibition in zebrafish larvae. To increase neutrophil density, <i>tg(lyz</i>:<i>DsRed)</i> embryos were injected with the <i>gcsfa</i> over-expressing plasmid at one cell stage. At 2 dpf, larvae were treated with VAS2870 to inhibit NOX enzyme. Then, NOX incompetent larvae were infected with <i>E</i>. <i>coli</i>-GFP for monitoring of the survival. (B) <i>Tg(lyz</i>:<i>DsRed)</i> larvae were imaged 5 h after treatment with DMSO or VAS2870 using fluorescence microscopy. The plot shows quantification of total neutrophils in indicated conditions (horizontal lines indicate mean number of neutrophils ± SEM, from two independent experiments, ANOVA with Tukeys’ post-test, <i>**p<0</i>.<i>01 and ***p<0</i>.<i>001</i>). (C) One hour after treatment (at 2 dpf) larvae were infected in the notochord with <i>E</i>. <i>coli</i>-GFP. Survival curves of larvae in indicated conditions from 0 to 3 dpi (<i>N</i>_{<i>larvae</i>} is indicated in the figure, log rank test, *<i>p>0</i>.<i>05</i>, ns: not significant).