<i>In vitro</i> ubiquitination of alpha-synuclein by different Nedd4-type ligases.

<p>Western blots of <i>in vitro</i> ubiquitination assays using different members of the Nedd4 family [250nM] with alpha-synuclein [600nM] (antibody Syn-1). (A) Comparing the ability of the Nedd4-type E3 ligases to ubiquitinate monomeric (mo.) or filamentous (fil.) alpha-synuclein indicates a general preference for filaments. (B) Ubiquitination assays of different Nedd4 family members with filamentous alpha-synuclein with or without the ubiquitin chain-type specific deubiquitinase Trabid as indicated above the panel. In each case the ubiquitin chains were sensitive to Trabid, implying similar linkages.