MMP9 and TP53 mRNA expression profiles in stratified cholesteatoma and control samples.

<p>Surgical samples were homogenized, total RNA was prepared and the cDNA generated was used as template for quantitative RT-PCR. Expression values were normalized to the PPIA gene. (A) Significantly elevated MMP9 expression is seen in recurrent cholesteatoma samples compared to nonrecurrent cholesteatoma samples or control skin samples. (B) There is no significant difference in TP53 expression levels among the groups. The mean and standard error of the mean values are shown. The significance level is (*) P-value < 0.05. The mean and standard error of the mean values are shown.