Binucleate apoptotic cells and MAP kinase.

<p>(A) <i>ced-1(e1735)</i> gonad at 48 hrs immunostained for activated MAP kinase (red, anti-dpMPK-1) and F-actin (green, phalloidin). The boxed region contains a binucleate apoptotic cell (arrow), as shown at higher magnification in panel A’ (the nuclear envelope is shown in magenta, anti-NPP-9). Note that this cell is located before the peak of activated MAP kinase, although most apoptotic cells (bracketed region in panel A) occur after the peak. Additional examples of binucleate apoptotic cells and dpMPK-1 are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007417#pgen.1007417.s008" target="_blank">S7 Fig</a>. (B and C) Cell deaths in a <i>nos-3(oz231); mpk-1(ga117)</i> null gonad (panel B), and in a <i>cep-1(RNAi); nos-3(oz231); mpk-1(ga117)</i> gonad (panel C). The presumptive apoptotic cells are small, have closed ring channels, and have compacted chromatin. Panel C’ shows an orthogonal view of the rod indicated in panel C (arrow). Bars = 20 microns (A), 5 microns (A’, B-C).