Kinesin is required for mitochondrial exit.

<p>(A) <i>ced-1(e1735); unc-116(RNAi)</i> adult gonad with a large cluster of apoptotic cells (X). The F-actin and PGL-1 signals are imaged in the same channel (red) to identify apoptotic cells by their small size and lack of PGL-1 on P granules (lower left panel). The top right panel shows mitochondria (green, ATP synthase beta) visible in the same, single optical, plane. Panel A’ shows the outlines of the apoptotic cells drawn on a 3 micron projection of the mitochondrial signal; the projection is comparable to the diameter of an apoptotic cell, and reveals most of the mitochondria within a cell. Note that the projection shows a large cluster of mitochondria in all but one of the apoptotic cells (outlined). (B) Germ cells in a <i>miro-1(tm1966)</i> null mutant, stained as for panel A. The arrow indicates the plasma membrane of an apoptotic cell (X), which lacks mitochondria. An adjacent, non-apoptotic cell is outlined for comparison. The DNA panel is a projection showing there is little or no chromatin compaction in the apoptotic cell. Thus, the apoptotic cell is at an early stage, and is unlikely to have contained, but degraded, any mitochondria. (C) Video sequence of an apoptotic cell in a <i>miro-1(tm1966)</i> null mutant expressing reporters for membranes (red) and for mitochondria (green, COX-4::GFP). Note that the exiting mitochondria (arrow) are aligned radially with respect to the gonad axis, similar to exiting mitochondria in wild-type apoptotic cells. (D) Germ cells in a <i>ced-1(e1735); klc-1(RNAi)</i> adult. The apoptotic cells (X) are outlined as for panel A. Note that all of the apoptotic cells contain clusters of mitochondria. (E) Video sequence of apoptosis in a <i>klc-1(ok2609)</i> null mutant; the complete video is provided as <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007417#pgen.1007417.s017" target="_blank">S4 Video</a>. Panel E’ shows the apoptotic cell at high magnification; the ring channel is open at the first time point shown, but closes by 18 mins. Bars = 5 microns (A,D), 2.5 microns (B-C, E). Fluorescent reporter: (C, E) JJ2586 + OD70.