CCR8 deficiency does not grossly perturb thymic architecture or alter thymocyte subset localization.

<p>(A) Representative immunostaining of <i>Ccr8</i>^<i>+/+</i> and <i>Ccr8</i>^<i>-/-</i> thymic cryosections with antibodies against pan-cytoKeratin (Pan-cK; cyan) and Keratin 5 (K5; magenta), to reveal cortical and medullary epithelium, respectively. Bar 1mm. (B) Ratios of cortical to medullary areas in <i>Ccr8</i>^<i>+/+</i> and <i>Ccr8</i>^<i>-/-</i> thymic cryosections were calculated from immunofluorescence images as in (A). Mean + SEM are shown for 5 <i>Ccr8</i>^<i>+/+</i> mice and 7 <i>Ccr8</i>^<i>-/-</i> mice; n.s. not significant (Unpaired Student’s <i>t</i> test). (C) Immunostaining of <i>Ccr8</i>^<i>+/+</i> and <i>Ccr8</i>^<i>-/-</i> thymic cryosections with antibodies against keratin 5 (K5; cyan), to mark medullary epithelial cells, as well as CD4 (magenta) and CD8 (green) to distinguish DP, CD4SP, and CD8SP thymocyte subsets. The white dashed line separates cortex (C) from medulla (M). Bar 100μm. Representative of three mice per group.