PKO and rho⁰ MEFs have similar cell growth, cell cycle and metabolic gene expression profiles.

<p>(A) Scatterplot of mitotic gene expression changes between PKO (y-axis) and rho⁰ (x-axis) MEFs with respect to TM6 MEFs (calculated as log₂ fold-change) (n = 3 biological replicates per line, 12 total). Linear regression lines were fit and Pearson (top value) and Spearman (bottom value) correlation coefficients were calculated with accompanying <i>P</i> values calculated using two-tailed <i>t</i> significance tests. Gene sets were derived from KEGG database cell cycle ID MMU04110. (B) Growth (biomass accumulation) rates for TM6, rho⁰, PKO-1, and PKO-6 cells were quantified by live cell interferometry (TM6 n = 308, rho^0: n = 233, PKO-1: n = 303 PKO-6: n = 364). (Median = red stripe, 95% confidence interval = pink region ± SD = purple region. (C) Proliferation of TM6, rho⁰, PKO-1 and PKO-6 MEFs by manual cell counting (n = 3, ± SD). (D) Stacked barplots of phases of the cell cycle in percentage distributions for TM6, rho⁰, PKO-1, and PKO-6 MEFs (n = 3, ± SD). (E) Representative flow cytometry profiles for TM6, rho⁰, PKO-1, and PKO-6 MEFs. DNA was stained with propidium iodide.