Opsonic killing of multiple intracellular pathogens by antibody to PNAG, complement (C’) and PMN depends on infected-cell surface expression of PNAG and is associated with release of LDH.

<p><b>A:</b> Killing of 5 different intracellular bacterial pathogens by monoclonal or polyclonal antibody (10% concentration) to PNAG plus PMN and C’ was markedly reduced following treatment of infected cells with Dispersin B (circles) to digest surface PNAG compared to treatment with the control enzyme, Chitinase (squares). Symbols represent indicated bacterial target strain. Horizontal bars represent means, and error bars show the 95% C.I. Symbols showing <0% kill represent data wherein the cfu counts were greater than the control of PNAG MAb + PMN + HI C’. P values: paired t-tests comparing percent intracellular bacteria killed with each antibody/antiserum tested after Chitinase or Dispersin B treatment. <b>B:</b> Opsonic killing is associated with maximal LDH release from infected cells in the presence of antibody to PNAG, C’ and PMN. Bars represent means from 5 different intracellular pathogens, error bars the 95% C.I., overall ANOVA P value by one-way repeated measures ANOVA, pair wise comparisons determined by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli.