Features of uORFs with different translational breadths and efficiencies.

<p>(A-C) Box plots for Kozak score around uAUGs (A), the distance from a uAUG to the downstream cAUG (B), and the phyloCSF score (C) for each class of uORFs (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001). The raw data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003903#pbio.2003903.s067" target="_blank">S1 Data</a>. (D) The relationship between phyloCSF (x-axis) and log₂(TE) (y-axis) of ribosome-associated uORFs in each of the 12 samples. The ribosome-associated uORFs were ranked with increasing phyloCSF and divided into 200 bins of equal size. Median phyloCSF and median log₂(TE) in each bin were displayed in the plot and used to calculate Spearman’s correlation coefficient. In each sample, only uORFs with mRNA RPKM ≥ 1 and TE ≥ 0.5 were used in the analysis. The raw data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003903#pbio.2003903.s068" target="_blank">S2 Data</a>. The raw data for panels (A-E) can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003903#pbio.2003903.s067" target="_blank">S1 Data</a>. cAUG, AUG start codon of CDS; DMSO, dimethyl sulfoxide; RPKM, reads per kilobase of transcript per million mapped reads; TE, translational efficiency; uAUG, AUG start codon of uORF; uORF, upstream open reading frame