Akirin regulates multiple <i>nlp</i> genes in the epidermis.

<p><b>A.</b> Confocal images of IG1485 transgenic worms expressing an <i>akir-1p</i>::<i>gfp</i> reporter gene showing epidermal and neuronal expression of GFP. The lateral epithelial seam cells are indicated by the arrowheads. Much of the fluorescence in the head and tail comes from neurons, seen more clearly in the right panel. Scale bar 50 μm. <b>B.</b> Ratio of green fluorescence (GFP) to size (TOF) in <i>rde-1</i>(<i>ne219</i>);<i>wrt-2p</i>::<i>RDE-1</i> worms that are largely resistant to RNAi except in the epidermis, carrying the array <i>frIs7</i>, treated with RNAi against the indicated genes and infected by <i>D</i>. <i>coniospora</i>. The black bar represents the mean value for (from left to right), n = 135, 49, 155, 102, 130, 94; **** p<0.0001, Dunn’s test. <b>C.</b> Quantitative RT-PCR analysis of the expression of genes in the <i>nlp-29</i> cluster in <i>rde-1</i>(<i>ne219</i>); <i>wrt-2p</i>::<i>RDE-1</i> worms treated with RNAi against the indicated genes and infected by <i>D</i>. <i>coniospora</i>; results are presented relative to those of uninfected worms. Data (with average and SD) are from three independent experiments (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007494#pgen.1007494.s002" target="_blank">S2B Fig</a>). **, p < 0.001; *, p < 0.01; 1-tailed ratio paired t test.