Complementation of the <i>ctr1</i>⊿<i>ctr2</i>⊿ <i>S</i>. <i>cerevisiae</i> mutant by <i>ZmCOPT</i> genes on YPEG media and the growth patterns of the wild yeast cells transformed with <i>ZmCOPT</i> genes on YPD media with excess Cu.

<p>A: <i>ctr1</i>⊿<i>ctr2</i>⊿ mutant was transformed with vector pGADT7 (negative control) or with <i>ZmCOPTs</i> expression vector. Wild yeast cells were positive control. Cells were grown on YPD or YPEG media for 3 to 6 days at 30°C. B: The density of cells containing <i>ctr1</i>⊿<i>ctr2</i>⊿mutant transformed with <i>ZmCOPTs</i> and wild yeast cells in liquid YPEG (OD₆₀₀) was monitored every 2 h for the first 12 h and at 24 h. Data are average values of three independent experiments and bars indicate mean ± SD. C: The growth patterns of the wild yeast cells and wild yeast transformed with <i>ZmCOPTs</i> expression vector or empty vector pGADT7 on YPD media supplemented with Cu (0 mM, 0.5 mM, 1 mM CuSO4) for 3 to 6 days at 30°C.