MOESM1 of The agr quorum sensing system in Staphylococcus aureus cells mediates death of sub-population

Additional file 1: Figure S1. Hemolysis negative cells arise in S. aureus Newman. Five parallel cultures of WT were passaged in TSB for 24 days. Every second day suitable dilutions were plated out on TSB agar with 5 % calf’s blood and each colony was scored for hemolysis by comparing to WT freshly inoculated from the freeze stock. Zones of hemolysis smaller than ~0,5 mm were scored as hemolysis negative. Figure S2. RNAIII overexpression with pTXΔRNAIII. RT-qPCR was used to measure expression of RNAIII in S. aureus Newman carrying vector (pTXΔ) or RNAIII overproducing plasmid (pTXΔRNAIII) after 6 hours of growth in TSB. Data represent three biological replicates and are shown as mean ratios normalized to a run calibrator of genomic DNA. Error bars represent the standard deviation. Figure S3. Lysed bacteria supports growth. WT cells were grown in diluted 0.1xTSB supplemented with increasing amounts of bacterial lysate, and growth was measured in a Bioscreen at OD600. The experiment was performed with biological triplicates for each condition and the data represent the mean OD600 and standard deviation. Table S1. Strains and plasmids used in this study. Table S2. Oligonucleotides used in this study