SclB functions downstream of FluG.

<p>A) Indicated strains were point inoculated and grown for 3 d in light or dark at 37°C. <i>sclB</i> OE does not rescue Δ<i>fluG</i> suggesting that <i>fluG</i> is epistatic to <i>sclB</i>. B) qRT-PCR of RNA, which was extracted from cultures grown under submerged conditions on a rotary shaker at 37°C and harvested after 24 h (vegetative) or subsequently shifted onto solid MM plates and grown for 24 h at 37°C in light (asexual). The <i>fluG</i> expression is downregulated in the absence of <i>sclB</i> during vegetative growth and upregulated during asexual growth (*** <i>P</i><0.001). C) qRT-PCR of vegetatively grown cultures of Δ<i>fluG</i> shows that <i>sclB</i> expression is decreased in the absence of <i>fluG</i>. Normalized against <i>h2A</i>, <i>gpdA</i> and <i>15s rRNA</i> (*** <i>P</i><0.001).