IL-27 inhibits T-bet expression in antiviral CD4 T cells with a cytotoxic phenotype.

<p>WT and <i>Il27ra</i>^<i>-/-</i> mice were infected with 1*10⁴ PFU MCMV and expression of the transcription factors T-bet and Eomes was analyzed in CD11a⁺CD49d⁺ CD4 T cells by mean fluorescence intensity (MFI) at day 21 p.i. (A-B) T bet (A) and Eomes (B) expression in KLRG1^- and KLRG1⁺ cells from WT animals compared to KLRG1⁺ cells from <i>Il27ra</i>^<i>-/-</i> mice. (C-D) T-bet (C) and Eomes (D) expression in GrzA^- and GrzA⁺ cells from WT animals compared to GrzA⁺ cells from <i>Il27ra</i>^<i>-/-</i> mice. As a positive control for Eomes staining, CD11a⁺CD49d⁺ CD8 T cells were included in histograms depicting Eomes expression (dotted line). One representative of 2 independent experiments with n = 5 mice per group. * p < 0.05, ** p < 0.01.