Live imaging of renal organoids using light sheet fluorescence microscopy.

<p>A) Detailed views of representative single focal plane images showing Wt1-GFP cells (green) grouped around a ureteric bud structure labelled with PNA (red). Over the depicted period of 23.5 hours, Wt1-GFP cells were entirely absent from the ureteric bud. Every cell surrounding the ureteric bud structure appeared to express GFP. Scale bar 25 μm. B) Three-dimensional segmentation of the ureteric bud structure shown in A). The ureteric bud segment is indicated with an arrow. The rod-shaped segment at the bottom is a piece of dust integrated in the organoid. Scale bar: 30 μm. C) represents the volume increase of the ureteric bud structure throughout the time series. D) shows that the cell diameter does not change over time. E) shows a comparison of the track displacement histograms of the two time series discussed in this and the previous figure, which are similar.