Glomerular structures stained positively for podocyte markers indicating phenotypic maturity in re-aggregated embryonic renal organoids after 6 days of culture.

<p>Two whole organoids (A-G and H-N) were imaged in the light sheet microscope and are depicted as maximum intensity projections (A, H) and respective selected single focal planes (B, I). Glomerular structures are highlighted with a dashed square in the single focal plane, and detailed images are shown in C-G and J-N respectively. The Wt1-GFP structure in C also stained positive for nephrin (E), and the Wt1-GFP structure in J stained positive for synaptopodin (L). Cytokeratin highlights the various ureteric bud foci, and PNA and laminin the basement membranes of the ureteric bud and nephric tubules. The sample shown in H-N contains fluorescent beads as reference points for multi view reconstruction of the dataset. The organoids were not cleared. Scale bars in A, B, H and I: 100 μm, G: 10 μm, N: 25μm.